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. 1999 Jul 20;96(15):8483–8488. doi: 10.1073/pnas.96.15.8483

Figure 1.

Figure 1

Constructs of the gene-switch-TGFβ1 transgenes. (A) The regulator transgene was inserted into the ClaI site of the ML expression vector. The GLVPc regulator comprises the DNA binding domain of the GAL4 activator (residues 1–93), the ligand binding domain of the truncated human progesterone receptor, which lacks 19 amino acids at the C terminus (hPRB914), and the herpes simplex virus VP-16 transactivation domain (residue 411–487). Primers 1 and 2 were used in PCR to identify the GLVPc transgene. (B) The target transgene, the porcine TGFβ1S223/225 cDNA, was inserted into a TK promoter with four copies of the 17-mer GAL4 consensus binding sequence situated upstream and the SV40 Poly(A) region at the 3′ terminal of the TK promoter. Primers 3 and 4 were used in PCR to identify the TGFβ1 transgene.