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Expression of p21waf1 mRNA in the control H460 cell line (lane 1), in FHIT transfected clones 2.I and 2.3 (lanes 2 and 3), and in the small-cell lung cancer cell line AFL, which lacks endogenous Fhit expression (lane 4). Total RNA (20 μg) was analyzed by Northern blotting, with successive hybridization to p21waf1 and GAPDH cDNA probes.
