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. 2003 Sep;62(9):851–858. doi: 10.1136/ard.62.9.851

Figure 4.

Figure 4

Cytometric characterisation of peripheral CD19+ B cells in a child with SLE (donor No 2). (A) Viable peripheral mononuclear cells of a patient with SLE, gated according to scatter and propidium iodide exclusion, were tested for CD19 and CD27 expression. CD19+ B cells were gated for further analysis as indicated. (B) Staining of CD27 versus forward light scatter to analyse differences in cell size. (C-I) CD19+ B cells, as gated in (A), were counterstained for HLA-DR, CD20, CD38, CD95, IgM, IgG, or IgD, respectively. Formaldehyde fixed cells were gated according to scatter and CD19-PE staining and counterstained for CD27 and for Igκ and Igλ light chains, without (J) or with (K) permeabilisation of the cell membrane by the use of saponin.