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. 1999 Jul 20;96(15):8511–8515. doi: 10.1073/pnas.96.15.8511

Figure 4.

Figure 4

14-3-3 binding inhibits ASK1-induced apoptosis. HeLa cells were cotransfected with an eGFP expression vector and test plasmids as indicated. Thirty-six hours posttransfection, cells were subjected to analysis. (A) ASK1-transfected cells show nuclear morphology characteristic of apoptosis, as determined by DAPI staining and fluorescence microscopy. Representative cells with apoptotic nuclei are indicated by arrows. (B) ASK1-induced apoptosis is modulated by 14-3-3 binding. Top shows the quantitation of apoptotic cell death by using nuclear morphology. The ratio of apoptotic transfected cells to total counted transfected cells is indicated above each bar. Middle shows expression levels of HA–ASK1 and Flag-tagged 14-3-3ζ proteins as determined by immunoblot. Bottom depicts the DNA integrity of transfected cells. Total DNA from the transfected samples was isolated, and an equal amount of DNA from each was separated on a 1.5% agarose gel.