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Tailless HLA-A2 associates with US2 and US11 in vitro. Full length and tailless A2 mRNAs were translated in a microsome-supplemented in vitro translation system together with β2m, HLA-DR1α, and US2 or US11 mRNA in the presence and absence of 1 mM DTT, as indicated. A portion of the total translation mixture was loaded directly (left lanes). The remainder of the microsomes was lysed and subjected to immunoprecipitation with W6/32 antibody (right lanes).
