Table 1.
Activating domain fusion | LexA DNA-binding domain fusion | Relative luciferase activity* |
---|---|---|
Ste20p (1–495) | Vector | 1.0 |
Ste20p (1–495) | Cdc42p | 18.6 |
Ste20p (1–495) | Hsl7p | 7.4 |
Vector | Cdc42p | 1.4 |
Vector | Hsl7p | 1.5 |
Ste20p (1–495) | Hsl7p (with overexpression of CDC42) | 1.7 |
Ste20p (1–495) | Cdc42p (with overexpression of HSL7) | 3.1 |
The relative luciferase activities are normalized to that for Ste20p-vector.