Figure 1 .

Messenger RNA expression of PAD isotypes in PBMCs. Total PBMCs from a healthy person were fractionated with MACS magnetic beads coated with either anti-CD3 (T cells), anti-CD14 (monocytes), anti-CD19 (B cells) or anti-CD56 (NK cells) for RNA isolation. Expression of PAD was analysed by RT-PCR. PAD1 and 3 were not detectable in any of the PBMC fractions. For PAD2 and PAD4, specific PCR products were produced in all fractions. In the case of CD3, CD19, and CD56, the cells that did not bind to the beads (CD3– CD19–, CD56–) showed higher expression than the cells that did bind (CD3+, CD19+, CD56+). In contrast, the cells that were bound by the anti-CD14 coated beads (CD14+, monocytes) showed higher PAD2 and PAD4 expression than the cells that did not bind the anti-CD14 coated beads (CD14–, all but the monocytes) as indicated by white squares. ß-Actin served as a control for mRNA input. Cloned cDNAs served as positive PCR controls, (no positive control included for ß-actin), PCR without template as negative control.