Figure 2 .

PAD4 mRNA is only expressed in monocytes, not in macrophages. RNA expression of PAD2 and PAD4 in freshly isolated monocytes (mono) from peripheral blood and in ex vivo differentiated macrophages (macro) of patients with RA (RA1–RA3) and healthy controls (HC1–HC3) was analysed by RT-PCR. ß-Actin served as a control for mRNA input, α₂-macroglobulin (α-2-m) expression was analysed as a control for macrophage differentiation. Cloned cDNAs served as positive PCR controls, (no positive control included for ß-actin), PCR without template as negative control.