Figure 7 .

Vimentin is specifically citrullinated in calcium stimulated macrophages. Immunoprecipitated vimentin from monocytes (A) and macrophages (B) from a healthy control (HC) and a patient with RA, treated for 0, 15 minutes, 60 minutes, or overnight (o/n) with 1 µM ionomycin in the presence of 2 mM CaCl₂, was stained with anti-MC antibodies. In vitro citrullinated human fibrinogen (25 ng) is included on the western blot as a positive control. Molecular weight markers (kDa) are indicated on the left.