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. 2004 May;63(5):578–582. doi: 10.1136/ard.2003.012948

Figure 1 .

Figure 1

Reverse transcriptase polymerase chain reaction (RT-PCR) amplifications representing interferon regulatory factor-1 (IRF-1) mRNA and 18 S rRNA in patients and controls. (A) Group A, lanes 1–7: seven myelodysplasia patients with autoimmune manifestations. (B) Group B, lanes 1–7: seven myelodysplasia patients without autoimmune manifestations. (C) Group C, lanes 1–5: five patients with systemic vasculitis. (D) Group D, lanes 1–7: seven normal individuals. Myelodysplasia patients from group B have decreased levels of full length IRF-1 mRNA expression in association with exon skipped products, as in case 1 and 4 (arrows). On the other hand, myelodysplasia patients from group A express the full length of IRF-1 mRNA (a 1006 bp product), without exon skipped products. The control groups (C and D) also show expression of the full length of IRF-1 mRNA. M, 100 bp marker (Gibco).