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. 2004 Sep;63(9):1104–1112. doi: 10.1136/ard.2003.016691

Figure 2.

Figure 2

 FACS analysis establishes binding of ETI-104 to RBCs of NHV. (A) Binding of ETI-104 to RBCs of each individual was confirmed by FACS analysis in vitro (goat antimouse for detection of the murine CR1 antibody and the PicoGreen DNA component of ETI-104). (B) RBCs from NHV 103 were bound in vitro to a saturating amount of Alexa-488 labelled anti-CR1 monoclonal antibody (mAb; 7G9) in the presence of varying concentrations of AHP. Increasing concentrations of AHP blocked 7G9 binding to the cells, demonstrating that AHP binds specifically to CR1. Thus, 250 ng AHP was not sufficient to block all available CR1. (C) RBCs from NHV 103 were stained after drug infusion to detect binding of the murine CR1 mAb (antimouse) and DNA (PicoGreen) components of ETI-104. The average of the geometric mean fluorescence intensity after subtracting non-specific fluorescence is given. The pattern of staining was similar in the other 10 NHV. All values were measured in duplicate.