Figure 2.

 Effects of OSM and TNFα on the expression of ADAMTS aggrecanases in chondrocytes. Bovine nasal or human articular chondrocytes were cultured until 80–90% confluence and then serum starved overnight. Cells were stimulated with OSM, TNFα alone or in combination for 24 hours. Total RNA was isolated, reverse transcribed, and the resulting cDNA used in a separate real time PCR with specific primers for ADAMTS-1, -4, -5, -8, -9, or -15. No signal was detected for ADAMTS-8 in human chondrocytes with any treatment. Results were normalised to either 18S (for bovine) or GAPDH (for human) and are presented graphically as relative mRNA levels.⁴⁸ The data shown are representative of two separate chondrocyte preparations.