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LacZ mRNA in rat ankle joints after intra-articular injection of rAAV2 and rAAV5. Total mRNA was extracted from crushed ankle joints and cDNA was synthesised. PCR was performed using specific primers for the LacZ gene. GAPDH was used as internal control. To correct for differences in GAPDH mRNA, LacZ/GAPDH ratios are displayed as measured by computer assisted gel quantification. The uninjected left joints as well as the control joints did not show LacZ expression.
