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Kinetics of the NO effect (SNP) on chondrocyte apoptosis. Cells (5x105 normal human chondrocytes), treated with SNP at different concentrations for 12, 24, and 48 hours were fixed in 70% ethanol at 4°C, then washed and incubated with RNase and PI for 15 minutes at room temperature in the dark. Data are expressed as a percentage of apoptotic (hypodiploid) nuclei. The graph shows that SNP dose dependently induces chondrocyte apoptosis.
