Figure 5.
The effect of NO on mitochondrial depolarisation is reversible. Cells (5x105 normal human chondrocytes) were treated with 2 mM SNP for 24 hours. The medium was then removed and cells were washed, and new medium without SNP was added. After 24 hours, cells were analysed by flow cytometry on a FACScan (Becton and Dickinson, Mountain View, CA). The proportion of cells with mitochondrial depolarisation (group D) was quantified. The effect of SNP on Δψm was reversible. Shown are the results of six experiments at 5 hours. Bars show the mean (SD).