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. 2004 Sep 30;64(5):743–749. doi: 10.1136/ard.2004.025502

Figure 1.

Figure 1

 Anti-GPI-specific IgG purified from the serum of a patient with RA with high anti-GPI titre. (A) Samples from different purification steps, including the RA serum before purification, the purified total IgG fraction, and the purified anti-GPI IgG fraction, were tested for their binding to rabbit GPI (rGPI), human GPI (hGPI), bovine serum albumin, and glutathione-S-transferase as control antigens in an ELISA. Mouse K/BxN serum and normal serum from a healthy subject (NS), were included as positive and negative controls. The fraction containing the purified anti-GPI IgG preparation was highly specific for hGPI and did not react with the other control antigens. (B) Titration of the human anti-GPI IgG fraction showed positive GPI reactivity down to 0.06 µg/ml of IgG, comparable to the mouse K/BxN serum, whereas no reactivity was found in the remaining RA IgG fraction after affinity purification.