Skip to main content
. 2004 Oct 21;64(5):708–714. doi: 10.1136/ard.2004.025577

Figure 4.

Figure 4

 Expression of (A) cryopyrin, (B) ASC and pyrin mRNA in macrophage-like cells differentiated from healthy donor monocytes by 5 day culture in M-CSF and serum. Cells were treated with LPS (10 ng/ml), TNFα (50 ng/ml), or IL1ß (2 ng/ml) for 18 hours before real time qPCR analysis. Results are from three donors and expressed as GAPDH normalised REU as in the legend to fig 1. *p<0.05 compared with control, untreated cells by single ANOVA, and Dunnett's post hoc test. The cryopyrin message was induced by LPS and TNFα, whereas ASC message levels decreased under the same conditions. Pyrin was only raised by LPS but not by either cytokine. The IL1ß message was significantly up regulated by LPS and TNFα (A), whereas IL1ß itself up regulated MMP-9 expression (see text), indicating macrophage activation in all cases.