Skip to main content
. 2004 Nov 11;64(6):865–870. doi: 10.1136/ard.2004.028845

Figure 3.

Figure 3

 Induction of nuclear DNA fragmentation of CD68+ cells by CD64-CaMi. DNA content of CD68– lymphocytic and CD68+ monocytic cells was stained with PI and measured by flow cytometry (FACS). (A) Representative analysis showing that incubation of SFMC with CD64-CaMi (10–8 mol/l for 24 hours) results in a strong increase in apoptotic CD68+ cells with reduced nuclear DNA content owing to DNA fragmentation (from 3.5% to 92.1% of CD68+ cells). (B) On average (n = 3, 10–8 mol/l for 24 hours), a mean (SD) change from 4.2 (0.2)% apoptotic macrophages in control culture to 57.5 (18.5)% upon culture with CD64-CaMi (p<0.05) was seen. Apoptotic cell death of CD68– lymphocytic cells was not significantly changed (8.2 (4.0)% to 13.1 (8.9)%). *Significant cell death of CD68+ cells (p<0.05).