Abstract
In the present study, we infected B-cell (and antibody-)-deficient transgenic mice with the Giardia lamblia clone GS/M-83-H7. These animals were inhibited in intestinal anti-Giardia immunoglobulin A (IgA) production and could not resolve the parasite infection, and antigenic diversification within the respective parasite populations occurred in an unusually slow manner. These findings indicate an important immunological function of local IgA antibodies which promotes antigenic variation of the parasite and is involved in control of the parasite infection.
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Selected References
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- Adam R. D., Aggarwal A., Lal A. A., de La Cruz V. F., McCutchan T., Nash T. E. Antigenic variation of a cysteine-rich protein in Giardia lamblia. J Exp Med. 1988 Jan 1;167(1):109–118. doi: 10.1084/jem.167.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Aggarwal A., Merritt J. W., Jr, Nash T. E. Cysteine-rich variant surface proteins of Giardia lamblia. Mol Biochem Parasitol. 1989 Jan 1;32(1):39–47. doi: 10.1016/0166-6851(89)90127-8. [DOI] [PubMed] [Google Scholar]
- Bosma G. C., Custer R. P., Bosma M. J. A severe combined immunodeficiency mutation in the mouse. Nature. 1983 Feb 10;301(5900):527–530. doi: 10.1038/301527a0. [DOI] [PubMed] [Google Scholar]
- Bründler M. A., Aichele P., Bachmann M., Kitamura D., Rajewsky K., Zinkernagel R. M. Immunity to viruses in B cell-deficient mice: influence of antibodies on virus persistence and on T cell memory. Eur J Immunol. 1996 Sep;26(9):2257–2262. doi: 10.1002/eji.1830260943. [DOI] [PubMed] [Google Scholar]
- Byrd L. G., Conrad J. T., Nash T. E. Giardia lamblia infections in adult mice. Infect Immun. 1994 Aug;62(8):3583–3585. doi: 10.1128/iai.62.8.3583-3585.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Faubert G. M. The immune response to Giardia. Parasitol Today. 1996 Apr;12(4):140–145. doi: 10.1016/0169-4758(96)10004-1. [DOI] [PubMed] [Google Scholar]
- Gottstein B., Deplazes P., Tanner I. In vitro synthesized immunoglobulin A from nu/+ and reconstituted nu/nu mice against a dominant surface antigen of Giardia lamblia. Parasitol Res. 1993;79(8):644–648. doi: 10.1007/BF00932506. [DOI] [PubMed] [Google Scholar]
- Gottstein B., Nash T. E. Antigenic variation in Giardia lamblia: infection of congenitally athymic nude and scid mice. Parasite Immunol. 1991 Nov;13(6):649–659. doi: 10.1111/j.1365-3024.1991.tb00560.x. [DOI] [PubMed] [Google Scholar]
- Hemphill A., Stäger S., Gottstein B., Müller N. Electron microscopical investigation of surface alterations on Giardia lamblia trophozoites after exposure to a cytotoxic monoclonal antibody. Parasitol Res. 1996;82(3):206–210. doi: 10.1007/s004360050096. [DOI] [PubMed] [Google Scholar]
- Kitamura D., Roes J., Kühn R., Rajewsky K. A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin mu chain gene. Nature. 1991 Apr 4;350(6317):423–426. doi: 10.1038/350423a0. [DOI] [PubMed] [Google Scholar]
- Milich D. R., Gershwin M. E. T cell differentiation and the congenitally athymic (nude) mouse. Dev Comp Immunol. 1977 Oct;1(4):289–298. doi: 10.1016/s0145-305x(77)80012-8. [DOI] [PubMed] [Google Scholar]
- Nash T. E., Aggarwal A. Cytotoxicity of monoclonal antibodies to a subset of Giardia isolates. J Immunol. 1986 Apr 1;136(7):2628–2632. [PubMed] [Google Scholar]
- Nash T. E., Herrington D. A., Levine M. M., Conrad J. T., Merritt J. W., Jr Antigenic variation of Giardia lamblia in experimental human infections. J Immunol. 1990 Jun 1;144(11):4362–4369. [PubMed] [Google Scholar]
- Nash T. E., Merritt J. W., Jr, Conrad J. T. Isolate and epitope variability in susceptibility of Giardia lamblia to intestinal proteases. Infect Immun. 1991 Apr;59(4):1334–1340. doi: 10.1128/iai.59.4.1334-1340.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nash T. Surface antigen variability and variation in Giardia lamblia. Parasitol Today. 1992 Jul;8(7):229–234. doi: 10.1016/0169-4758(92)90119-m. [DOI] [PubMed] [Google Scholar]
- Stäger S., Felleisen R., Gottstein B., Müller N. Giardia lamblia variant surface protein H7 stimulates a heterogeneous repertoire of antibodies displaying differential cytological effects on the parasite. Mol Biochem Parasitol. 1997 Mar;85(1):113–124. doi: 10.1016/s0166-6851(96)02818-6. [DOI] [PubMed] [Google Scholar]