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Effects of TNFα or IL1ß used alone at low and optimal concentrations and in combination on NF-κB nuclear translocation. Myoblasts (104 cells/cm2) were stimulated for 30 minutes with TNFα or IL1ß at the indicated concentrations. Cells were fixed in paraformaldehyde 4% and immunofluorescence staining was performed. Nuclear translocation was evaluated and confirmed by nuclear staining with Hoechst H33258 (data not shown). Incubation with 20% FCS medium was used as a positive control.
