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. 1999 Jul 20;96(15):8567–8572. doi: 10.1073/pnas.96.15.8567

Figure 1.

Figure 1

(A) Growth of HCY4 and HCY4 Cbk1Δ in the presence and absence of peptide AptC6. Strains were spotted onto Plates that contained galactose and 0, 25, 50, 100 nM α-factor. (a) HCY4 cbk1Δ + pJG4–4. (b) HCY4+ pJG4–4. (c) HCY4 cbk1Δ + AptC6. (d) HCY4 + AptC6. (B) Interactions of AptC6 with Cbk1 in strains HCY4 or HCY4ΔSte5. Interactions are scored by activation of the LexAop-lacZ reporter as judged by blue color on 5-bromo-4-chloro-3-indolyl β-d-galactoside plates. Upper, experimental. AptC6 is in the bait construct and Cbk1 is in the prey construct. Lower, controls. AptC6 is in the bait but pJG4–5, the control plasmid, expresses no prey. AptC6 bait construct gives low-level basal activation and blue color. (C) Interaction between Cbk1 and proteins involved in pheromone response. Upper, Cbk1 is expressed as a prey, with the indicated proteins expressed as baits. Lower, Cbk1 is expressed as a bait and the indicated proteins as preys. Note that, as a bait, Cbk1 directs significant basal transcription. Interactions with Ste5, Ste50, and Ste20 are visible as an increase in blue color above that caused by transcription from the bait.