Table 2.

Pheromone response panel

Prey	Gpa1	Ste4	Ste18	Ste20	Ste50	Ste5	Ste7	Ste11	Far1	Ste12	Fus3	Kss1	Cdc28	Cln1	Cln2	Cln3
Bait
C1	13	—	—	—	—	—	—	402	—	—	—	15	—	—	—	—
C2	—	—	—	—	—	—	—	—	—	—	—	—	—	—	—	—
C3	—	—	—	—	—	—	—	—	—	—	—	—	—	—	—	—
C4	—	—	—	—	2,135	—	—	—	—	—	—	—	—	—	—	—
C5	27	—	—	—	—	—	—	—	—	—	—	—	—	—	—	—
C6	—	—	—	—	—	794	—	—	—	—	—	—	—	—	—	—
C7	—	—	—	—	37	355	—	—	—	—	—	69	—	—	—	—
N1	—	—	—	—	—	—	—	—	—	—	—	—	—	—	—	—
N2	—	—	—	—	—	555	—	—	—	—	—	—	—	—	—	—
N3	—	—	—	—	—	253	—	—	—	—	—	—	—	—	—	—
N5	—	—	—	—	90	—	—	—	—	—	—	—	—	—	—	—
Ste50	—	—	—	—	1,308	395	317	2,220	—	—	—	—	—	—	—	—
Ste7	—	—	—	—	—	844	—	—	—	—	—	—	—	—	—	—
Ste11	—	—	—	—	—	478	—	—	—	—	—	—	—	—	—	—
Fus3	—	—	—	—	—	—	—	85	—	—	—	767	—	—	—	—

Interactions between aptamers and selected pheromone-response proteins. In this experiment, aptamers and Ste50, Ste7, Ste11, and Fus3 are expressed as baits, while potential interacting proteins are expressed as preys. Numbers represent Miller units (35) of β-galactosidase activity determined by liquid assay for interactions that produced blue color on 5-bromo-4-chloro-3-indolyl β-d-galactosidase plates. Activity was corrected by subtracting basal reporter activation by the bait in control strains that express the bait but that contain an empty prey vector (pJG4-5). Standard deviations for all interactions were <20%. Gpa1 (36) is the α-subunit G protein; it is also known as Sgc1 (37).