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Absolute cell number of CD4+ T cells expressing AND TCR+ and endogenous TCR chains increase in CTLA-4-deficient animals. Single cell suspensions were prepared from the spleen and lymph nodes and stained for CD4 and Vβ3, Vα11, and the absolute number of each subpopulation was determined. Each dot represents a single animal. AND TCR+ CTLA-4+/+, +/−, n = 6 (⋄); CTLA-4−/−, n = 5 (♦).
