Table 2.

Linearity of Western blotting data

Antibody	Specimen	Cell equiv. (relative)	Densitometric value*
Anti-Lyn	D1 lysate	1.0	1,800
		0.5	933 ± 44
		0.25	415 ± 36
			β     γ
Anti-PY	Anti-IgE ppt.	1.0	1,283 ± 16 2,979 ± 49
(4G10)	(FcɛRI)	0.5	588 ± 23 1,391 ± 86
		0.25	286 ± 20   730 ± 70

The linearity of responses to antibody PY20 (a peroxidase-conjugated anti-PY) and the anti-β antibody JRK have been published previously (40, 41). 

¹⁷⁸The experiment was performed on three independently run gels. Results are mean ± SD.