Table 3.

Variation with stimulation time and reproducibility of replicates

Assay	tstim, min	Clone	PY*		D1/D7†
			β	γ2	β	γ2
1	7.5	D1	0.294	0.770
					2.91	2.17
		D7	0.101	0.354
	15	D1	0.527	1.317
					3.11	2.95
		D7	0.169	0.446
	30	D1	2.060	4.368
					3.51	2.81
		D7	0.586	1.550
2	15	D1	0.371	0.724
					6.74	4.02
		D7	0.055	0.180
	15	D1	0.267	0.606
					2.64	3.42
		D7	0.101	0.177
	15	D1	0.414	0.391
					7.39	7.24
		D7	0.056	0.054
Mean ± SEM   4.3 ± 0.83.7 ± 0.6

¹⁷⁸The values shown are the corrected densitometry readings obtained from the Western blots using anti-PY, divided by the readings similarly obtained with the anti-β (JRK). 

^†As seen in Table 1, the ratio Lyn_rel/FcɛRI in D1 is currently 3 times greater than in D7.