Figure 1.

Results of MACS of LNC from mL9-immunized mice. LNC from mL9 peptide-immunized mice were restimulated in vitro with 6132A-PRO tumor cell lysate and then incubated with microbead-coupled anti-CD4 antibodies for separation into CD4-positive and -negative fractions by using MACS positive selection columns. After overnight incubation, cell aliquots were stained with anti-CD4 and anti-CD8 antibodies and analyzed by flow cytometry. The numbers in the quadrants indicate the percentage of total cells that the different cell population in each quadrant comprises. Shown is the analysis of experiment 3 shown in Table 2.