Figure 2.

Flow cytometric analysis for human CD45⁺, CD33⁺, and CD8⁺ cells in the marrow of transplanted mice. Bone marrow was obtained 70 days after transplant from mice receiving 1.5 × 10⁶ 10885 AML that had been cultured in either medium alone (control; Top), with clone DRN-11 (Middle), or with clone DRN-7 (Bottom), at a T cell/AML cell ratio of 10:1. Cells were analyzed by flow cytometry to detect those expressing human CD45 (Left); the dark solid line shows staining with an isotype control mAb and the light solid line, staining with the anti-human CD45 mAb. (Center) Dual staining for CD33 and CD45. (Right) Dual staining for CD8 and CD45.