Figure 5.

CD8⁺ minor H antigen-specific CTL clone DJG-24 specifically inhibits engraftment of 10827 AML cells in NOD/SCID mice. (A) Cytolytic activity of donor CD8⁺ CTL clone DJG-24, against EBV-LCL, phytohemagglutinin-stimulated T cells, fibroblasts, and AML cells from the HCT recipient. Data shown are for an effector-to-target ratio of 10:1. (B) Flow cytometric analysis of human CD45⁺ cell engraftment 8 weeks after transplantation in cohorts of mice that received 5 × 10⁶ 10827 AML cells that had been cultured for 24 hr in medium alone (Control) or with DJG-24 CTL at a T cell/AML cell ratio of 10:1. Each symbol indicates the engraftment measured in an individual mouse. The mean levels of engraftment are indicated by the horizontal bars. The P value refers to the comparison of engraftment in the DJG-24 group and control mice, as computed by the Mann–Whitney u test.