Figure 6.

CD8⁺ minor H antigen-specific CTL clones inhibit AML engraftment in NOD/SCID mice by direct recognition of SL-ICs. Cohorts of mice were transplanted with a mixture of AML cells from patient 10138 (HLA A1⁺, A3⁺, B8⁺, B14⁺) and patient 11720 (HLA A3⁺, B7⁺, B8⁺) that had been cultured in either medium alone (○; n = 5), with clone MRR-24 (□; n = 3), or with clone ATT-7 (▵; n = 5). Bone marrow was obtained 10 weeks later and examined by flow cytometry using an HLA A1-specific mAb to detect 10138 AML cells (Upper) and an HLA B7-specific mAb to detect 11720 AML cells (Lower). The mean levels of engraftment in each treatment group are indicated by the horizontal bars. P values refer to the comparison between the engraftment in the indicated treatment group and that in control mice, as computed by the Mann–Whitney u test.