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. 1999 Jul 20;96(15):8657–8662. doi: 10.1073/pnas.96.15.8657

Figure 1.

Figure 1

Schematic diagram of transcription factor fusions and the reporter gene constructs. TF1 vector. The enhancer/promoter from CMV drives expression of a bicistronic transcript in which the first cistron encodes a fusion of the rapamycin binding domain of FRAP (FRB) with the activation domain of the p65 subunit of NF-κB. The second cistron, translated from an internal ribosome entry site (IRES), encodes 3 copies of FKBP fused to the chimeric DNA-binding domain ZFHD1. The 3′ untranslated region consists of an intron and polyadenylation sequence (PAS) from the rabbit β-globin gene (RBG). Both fusion proteins contain an amino-terminal epitope tag (E) and nuclear localization signal (N). ZG1 vector. This target gene vector contains 12 repeats of the recognition site for ZFHD1 upstream of a minimal IL-2 promoter driving expression of hGH, followed by the polyadenylation signal of the simian virus 40 late gene. ZG2 vector. This vector contains two transcription units in series. The ZG1 cassette is followed by all the elements of the TF1 cassette except the IRES and DNA-binding domain fusion.