Figure 4.

Long-term rapamycin-induced hGH secretion in mice receiving i.m. injection of AAV. Immunodeficient BALB/c mice were injected i.m. with a total of 2 × 10¹¹ genome copies of AAV.TF1 and AAV.ZG2 in a 1:1 ratio. Rapamycin (5 mg/kg filled triangles, 1 mg/kg open triangles) or a rapamycin analog with 10-fold decreased activity (5 mg/kg open circle) was administered i.p. in single or multiple doses as indicated. Values of plasma hGH are mean ± SEM (n = 5). The lower limit of detection of the hGH assay is 50 pg/ml.