Effects of transfection of TF cDNA, containing full-length sequence or the cytoplasmic tail deletion sequence into human melanoma cell line HT144. Filled bars represent the levels of TF and empty bars represent the levels of VEGF. The values are the mean ± 1 SD of triplicate assays from three separate experiments. Wild-type (WT) HT144 cells are low TF and VEGF producers. TF transfectants containing the full-length coding sequence (TFc5d) produced increased levels of both TF and VEGF. In contrast, TF transfectants containing the cytoplasmic tail deletion sequence (TFTSIIIB1) produced very little VEGF, although TF antigen production (and procoagulant activity, data not shown) was increased. Levels of VEGF in the nontransfected HT144 (WT) and HT144 transfected with the vector alone (Vector) were too low to illustrate on the graph, as was true for the vertical lines indicating SD for both TF and VEGF production by the WT cells and the cells containing vector, and for VEGF production by the TFTSIIIB-transfected cells.