Figure 4.

Effects on VEGF transcription by transfection of TF cDNA, containing full-length sequence or the extracellular domain mutant sequence (Y¹⁵⁷ → A¹⁵⁷ and K¹⁵⁹ → A¹⁵⁹) into human melanoma cell line HT144. (a) A 2% agarose gel demonstrating RT-PCR products of VEGF-165 mRNA produced by transfectants with vector alone, TF sense, or TF^mut (157/159) plasmids. (b) Relative levels of VEGF mRNA production by transfectants with vector alone, TF sense, or TF^mut (157/159) plasmids. Relative levels of VEGF mRNA from each transfectant colony were determined by densitometric quantitation of RT-PCR products separated on 2% agarose gel as shown in a. Relative VEGF units were determined by dividing the intensity of densitometric values for VEGF by the intensity of densitometric values for GAPDH. Values of each bar graph represent mean ± 1 SD of at least seven separate transfectant colonies.