Table 1.
TF and VEGF production in clones of the HT144 cell line transfected with truncated TF cDNA
| Clone | TF, pg/106 cells | VEGF, pg/106 cells |
|---|---|---|
| HT144-TFTSIII-B1 | 36,400 | 123 |
| HT144-TFTSIII-B6 | 5,100 | 0 |
| HT144-TFTSIII-B9 | 613 | 0 |
| HT144-TFTSIII-B14 | 3,569 | 0 |
| HT144-TFTSIII-B51 | 424 | 0 |
| HT144-TFTSIII-2 | 1,392 | 9 |
| HT144-TFTSIII-20 | 6,121 | 81 |
| HT144-TFc5d | 27,367 | 17,252 |
| HT144-wild type | 506 | 0 |
| HT144-vector | 365 | 0 |
| Hs294T | 10,099 | 8,098 |
HT144-TFTSIII-B1, -B6, -B9, -B14, -B51, -2, and -20 are clones derived from the low TF- and VEGF-producing human melanoma cell line HT144, each of which was transfected with the truncated TF cDNA (TFTSIII). This TF cDNA lacks the coding region for the cytoplasmic tail serine residues, potential substrates for PKC (14). HT144-TFc5d is a clone derived from the HT144 cell line that was transfected with the full-length TF cDNA. The HT144 vector is derived from HT144 that was transfected with the vector alone. Hs294T is a constitutively high TF- and VEGF-producing human melanoma cell line. TF and VEGF levels were measured in duplicate by ELISA. The data are representative of three separate experiments.