Table 2.

Sensitivities of HIV-1_LAI, HIV-1_Ba-L, and HIV-2_EHO to various PIs, including JE-533 and JE-2147

Virus	Phenotype	Cells	IC50, μM
			RTV	IDV	APV	SQV	NFV	JE-533	JE-2147
HIV-1LAI	SI	PBMC*	0.047	0.019	0.019	0.0054	0.0090	0.015	0.044
HIV-1Ba-L	NSI	PBMC*	0.018	0.0056	0.014	0.0037	0.0056	0.034	0.024
HIV-1LAI	SI	MT-2†	0.041	0.019	0.046	0.023	0.0050	0.020	0.035
HIV-2EHO	SI	MT-2†	0.37	0.006	0.55	0.0036	0.0050	0.50	0.047

^*IC₅₀s were determined by using PHA-PBMCs exposed to HIV-1 (50 TCID₅₀ dose, 10⁵ PBMCs) and by using the inhibition of p24 Gag protein production as an endpoint on day 7 of culture. All assays were conducted in triplicate. The values shown are representatives of two or three separate experiments. 

^†MT-2 cells (2 × 10³) were exposed to 100 TCID₅₀ of HIV-1_LAI or HIV-2_EHO and were cultured in the presence of various concentrations of reverse transcriptase inhibitors and PIs, and the IC₅₀s were determined by using MTT assay on day 7 of culture. All assays were conducted in duplicate, and the values shown are representative of two or three repeated experiments.