Table 4.
In vitro selection and phenotypic susceptibility of HIV-1 passaged with JE-2147
| Virus | Amino acid substitutions | JE-2147 concentration when examined | IC50, μM* | Fold resistance† |
|---|---|---|---|---|
| HIV-1LAI | Wild-type | None | 0.031 | 1 |
| HIV-1JE-2147-P32 | I47V | 0.3 | 0.041 | 1 |
| HIV-1JE-2147-P35 | I47V/I84V | 0.3 | 0.064 | 2 |
| HIV-1JE-2147-P53 | L10F/I47V/I84V | 0.5 | 0.60 | 19 |
| HIV-1JE-2147-P73 | L10F/M46I/I47V/I84V | 1.6 | 0.86 | 28 |
*
Sensitivity of each viral preparation selected to JE-214X was determined by using MT-2 cells and the MTT assay.
†
Fold resistance relative to the IC50 against HIV-1LAI.