Figure 4.

Methylation profile of colorectal cancer. The methylation status of all type C MINT clones, as well as p16, THBS1, and hMLH1, was determined in a panel of primary CRC and adjacent mucosa. None of the normal tissues studied showed any degree of methylation for these genes. Also shown are representative type A clones (Right). Each column represents a separate gene locus (indicated on top). Each row is a primary CRC (samples above the bold solid line) or adenomatous polyp (below the bold solid line). Black rectangles, methylated tumor; white rectangles, unmethylated tumor. MSI denotes the presence of microsatellite instability and was not determined for the adenomas. In the Center are known genes methylated in CRC (p16, THBS1, hMLH1).