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. 1999 Jul 20;96(15):8687–8692. doi: 10.1073/pnas.96.15.8687

Figure 3.

Figure 3

Luciferase activity in Ascaris embryos: mRNA gene promoter and trans-splice acceptor DNA constructs. Reporter constructs (see A) were introduced into 32- to 64-cell Ascaris embryos. Error bars in B-D represent SEM (n = 4) for these representative experiments. C and D are independent experiments. Relative luciferase light units represent ≈ 15–25% of the total activity in the embryos. (A) Organization of luciferase constructs containing putative Ascaris promoters and a trans-splice acceptor. (B) Luciferase expression derived from eF-1-gamma (translation elongation factor 1-gamma), fert-1, and rRNA promoter sequences 48 hr after transfection. (C) Luciferase expression 20 hr after transfection with DNA constructs containing the Ascaris vacuolar ATP synthase subunit gene trans-splice acceptor. (D) Luciferase expression 48 hr after transfection with DNA constructs containing the Ascaris vacuolar ATP synthase subunit gene trans-splice acceptor.