Figure 7.

Binding of actin to profilin. The ³⁵S-labeled translation products of normal and mutant actin cDNAs were incubated with the GST-profilin-beads or GST-beads at 4°C for 2 hours. Recovered labeled actin from the beads was subjected to SDS/PAGE. The gel was dried and exposed to Fuji imaging plate, and radioactivity of the actin band was determined by using Bas 2000 (Fuji). The values after subtraction of those obtained by using GST-beads (<10%) are plotted. Open circle, control β-actin; closed circle, mutant β-actin.