Table 1.
No. | Primer sequence | Position, bp |
---|---|---|
DNA sequencing | ||
s-1 | TGTAAAACGACGGCCAGT | pKA1 primer |
s-2 | ATCGAGCACGGCATCGTCAC | 252–271 |
s-3 | GGACCTGACTGACTACCTCA | 590–609 |
s-4 | TGGAGAAGAGCTACGAG | 748–764 |
s-5 | ACCATGTACCCTGGCATTGC | 951–970 |
s-6 | CAAGTACTCCGTGTGGATCG | 1,046–1,065 |
s-7 | TGACTTAGTTGCGTTACACC | 1,178–1,197 |
s-8 | AACGGTGAAGGTGACAGCAG | 1,320–1,339 |
s-9 | TGCATTGTTACAGGAAGTCC | 1,433–1,452 |
s-10 | AACTTGAGATGTATGAAGGC | 1,656–1,675 |
a-1 | CACATAGGAATCCTTCTGAC | 184–203 |
a-2 | GAAGGTCTCAAACATGATCT | 403–422 |
a-3 | TGAGGTAGTCAGTCAGGTCC | 590–609 |
a-4 | CTCGTAGCTCTTCTCCAGGG | 744–763 |
a-5 | CGGAACCGCTCATTGCCAAT | 789–808 |
a-6 | GCAATGCCAGGGTACATGGT | 951–970 |
a-7 | GGTGTAACGCAACTAAGTC | 1,179–1,197 |
a-8 | GGACTTCCTGTAACAATGC | 1,434–1,452 |
a-9 | CCTTCATACATCTCAAGTTG | 1,655–1,674 |
a-10 | ATTAACCCTCACTAAAGGGA | T3 primer |
Genomic amplification and sequencing | ||
S-1 | CTCCATCATGAAGTGTGACGTG | 2,600–2,621 |
S-2 | ACATCCGCAAAGACCTGTAC | 2,623–2,642 |
A-1 | GTGTAACGCAACTAAGTCAT | 3,008–3,027 |