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Fig. 1. Specific silencing of PHD isoforms using siRNAs. Northern blot showing total RNA isolated from HeLa cells after 48 h of transfection with the indicated siRNAs (20 nM). Blots were hybridized with 32P- labelled probes specific for PHD1, PHD2, PHD3 or 36B4 (loading control). Inset shows localization of the PHD region targeted by the two independent sets of siRNAs (filled squares and double asterisks).
