Fig. 3. Elg1 is required for the DNA damage response. (A) Ten-fold serial dilutions of cultures of the indicated mutants were spotted on YPD, YPD containing 0.01% (v/v) MMS, 0.035% (v/v) MMS, 50 mM HU, or on YPD that was subsequently exposed to 100 J/m2 UV. Plates were incubated at 30°C for 2–3 days. (B) Logarithmically growing cultures of the indicated mutants were incubated in YPD containing 0.035% (v/v) MMS at 30°C. At the indicated times, samples were withdrawn and plated on medium lacking MMS to determine the number of viable cells. The percentage of viable cells relative to the number of viable cells at t = 0 is shown. Plots represent the average of three experiments, and error bars span 1 SD.