Fig. 4. Growth in tryptone-based liquid media. Growth curves of cultures in various test media (see Materials and methods) inoculated with stationary-phase bacteria bearing pGEM-empty (left panels), pGEM-WT (middle) or pGEM-S331P (right). Mean ± SD given (n ≥ 3, SD smaller than symbol when not visible). (A) Growth of cells in the T medium (open squares, broken line), TK5 (open triangles), TNa5 (open circles) and TS10 (open diamonds). (B) Growth of cells in T medium (pH 7.3, open squares, broken line), TH5 (pH 5.8, gray squares, only pGEM-S331P was measured), TK5 (pH 7.3, open triangles) and TK5H5 (pH 5.8, gray triangles). The pH variations of the cultures remained within ±0.1 U in the first 4 h. (C) Growth of cells before and after the addition of 10 mM KCl (black triangles), NaCl (gray circles) or none (open squares, broken line), at the logarithmic-phase of growth (arrows).