Table I. Random mutations on three key residues of Kch’s filter (G187, Y188 and G189) and their effect on the S331P GOF mutant phenotype.
Filter sequence |
||||||
---|---|---|---|---|---|---|
G187 |
Y188 |
G189 |
||||
Codon |
A.A. |
Codon |
A.A. |
Codon |
A.A. |
|
Wild type | GGC | G | TAT | Y | GGC | G |
Mutations that suppress the GOF phenotype | TGC | C | ||||
CAG | Q | |||||
GGC | G | |||||
ACT | T | |||||
CGC | R | |||||
ATA | I | |||||
AAA | K | |||||
CAA | Q | |||||
TCC | S | |||||
AGC | S | |||||
GAC | D | |||||
CTT | L | |||||
TTC | F | |||||
AGA | R | |||||
AAT | N | |||||
AGT | S | |||||
TCA | S | |||||
AAA | K | |||||
TCC | S | |||||
GCA | A | |||||
GAG | E | |||||
Do not suppress |
|
|
TTC |
F |
|
|
TAT | Y | |||||
TTC | F | |||||
TAT | Y | |||||
TTT | F |