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. 2003 Aug 15;22(16):4111–4120. doi: 10.1093/emboj/cdg411

graphic file with name cdg411f2.jpg

Fig. 2. Despite RssB binding, region α2.5 is not sufficient to trigger proteolysis. Cells expressing RpoS742::LacZ (A) or α2.5::LacZ (B) from pRH800 derivatives were grown in minimal medium (M9/0.1% glucose) to an OD578 of 0.6 and pulse-labeled with [35S]methionine. The pulse time was 2 min; chase times varied between 2 and 30 min. For immunoprecipitation, a polyclonal serum against β-galactosidase was used. Quantification of the data shown in (A) and (B) is given in (C), with closed symbols for RpoS742::LacZ and open symbols for α2.5::LacZ.