Fig. 7. RpoS165::LacZ binds to ClpX in vitro. Equimolar amounts of crude cell extracts of strains overexpressing either RpoS165::LacZ or RpoS165^Δ7–35::LacZ were incubated with 0.3 nmol ClpX in the presence or absence of ATP-γ-S and subject to co-immunoprecipitation, SDS–PAGE and protein staining as described in Materials and methods.