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. 1999 Jul 20;96(15):8711–8715. doi: 10.1073/pnas.96.15.8711

Figure 1.

Figure 1

The level of p21 protein in antisense p21-transfected Daudi cells after treatment with anti-IgM. Equal amounts (50 μg of total protein) of cell lysates from WT, control, and AS cells treated or untreated for 24 hours with 20 μg/ml anti-IgM were electrophoresed on a 12% SDS/PAGE gel and were transferred to poly(vinylidene difluoride) membranes. The p21 protein in each sample was detected with 1 μg/ml anti-p21 mAb followed by enhanced chemiluminescence detection. Values represent the relative induction of p21 as normalized to p21 protein levels in untreated WT cells as detected by densitometric scanning of the bands. A representative experiment of three is shown.