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Expression of mIgM on transfected Daudi cells. Cell surface expression of IgM was analyzed by flow cytometry of cells that were stained directly with FITC-conjugated IgG1 anti-human μ mAb (HB57). A total of 5 × 105 cells were stained for each sample with a preliminary-determined optimal concentration of mAb. The thin line indicates the background staining with a FITC-conjugated IgG1 anti-HER2 control mAb. A representative experiment of three is shown.
