Figure 3.
(A) Detection of pCBM-BSD DNA from parasites selected on 1 μg/ml (BS1), 2 μg/ml (BS2), or 5 μg/ml (BS5) of blasticidin S. Genomic DNA (prepared 7 weeks posttransfection) and DNA from the original plasmid (pCBM-BSD) were restricted with ScaI (S, which cuts once in the vector) or ScaI + DpnI (SD, with 15 pBluescript recognition sites), hybridized with a 32P-labeled pBluescript probe, and detected by autoradiography. A longer exposure of the autoradiograph is also shown. (B) Detection of pCBM-NEO DNA from parasites selected on 500 μg/ml G418 (G5). DNA was prepared as described for A. Untransfected 3D7 served as a control in both A and B.