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Plasmid copy number in G3 and G5 clones (Upper) and BS1, BS2, and BS5 (Lower). By using the genome size of 3 × 107 bp, the amount of NEO (800 bp) or BSD (390 bp) signal that would represent one copy per P. falciparum genome was calculated; 0.5 μg of DNA from 3D7 and the various transformants were used for comparison.
